Containers for preservation of bacteria cultures



NOV. 25, 1952 S N 26l9448 CONTAINERS FOR PRESERVATION OF BACTERIA CULTURES Filed Jan. 20, 1950 v INVENTOR. Gasiay [arsen Patented Nov. 25, 1952 CONTAINERS FOR PRESERVATION F BACTERIA CULTURES Gustav Larsen, Haslev, Denmark Application January 20, 1950, Serial No. 139,760 In Denmark January 21, 1949 4.- Claims.

The invention relates to improvements in containers for preservation of bacteria cultures, especially for the destruction of vermin such as rats and mice.

The object of the invention is to provide a container of the kind mentioned in which the bacteria culture may be preserved for a long time and easily be transplanted to a substratum for further development in view of subsequent use.

Another object of the invention is to provide a container which ensures an absolutely sterilized handling of the bacteria culture until it is taken out of the container for use.

A further object of the invention is to provide a container consisting of simple means to be produced at low costs, and which container does not necessitate intricate operations to be done when the bacteria are to be transplanted to the substratum.

These and further objects and advantages of the invention will be apparent from the following detailed specification.

The accompanying drawing illustrates a preferred embodiment of the invention.

In the drawing the container is a bottle I filled with a substratum 2, partly shown in dotted lines in the figure, e. g. a peptone-bouillon solution. Furthermore the bottle contains ampule 3 of a transparent glass having such internal tensions that it will crush to dust on being broken and having an external narrow pass 4. The ampule contains a bacteria culture, e. g. of the Salmonella typhi-murium well known for destruction of rats and mice.

Around the narrow pass 4 there is a thread or wire 5 which while keeping the container sterilized is taken out through the sealing of the container, which consists of a sterilized closing plug 6. In this way the bacteria culture can be preserved for several years, and shortly before its use the ampule 3 is broken by pulling the thread or wire 5 whereby the ends of the ampule first contact the shoulders I of the bottle and then the ampule is broken so that its content is poured out into the substratum 2 where it disperses and starts development without the container having been opened.

One day or two later the whole contents of the bottle are useful for destruction of rats and mice during the following month.

When breaking the ampule the contents of its two halves will quickly disperse in the substratum 2. While ordinary glass may be used, this dispersion will be accelerated if the ampule consists of glass having such internal tensions that it is crushed to dust when broken. This property may be imparted to the glass by quickly cooling the molten glass article as by dipping it in water.

It is to be understood that the form of my invention, herewith shown and described, is to be taken as a preferred example of the same, and

2 that various changes are possible within the scope of the claims, without departing from the spirit Of my invention.

What I claim and desire .to secure by Letters Patent is:

1. A container for the preservation of bacteria culture comprising a bottle having a narrow neck portion and a sterile closure, an ampule within said bottle enclosing a bacteria culture, said ampule having an external narrow pass in the middle thereof, a thread or wire extending through said closure and around said narrow pass in such manner as to hold said ampule in a transverse position near the narrow neck of the bottle so that when said thread is pulled said ampule will break against the neck of said bottle.

2. Container for the preservation of bacteria cultures, said container having a sterile closure, an ampule within said container enclosing a bacteria culture, a thread extending around said ampule and through said sterile closure in such manner that when said thread is pulled said ampule will contact the walls of said container and be broken.

3. Container for the preservation of bacteria cultures, said container having a sterile closure, an ampule within said container enclosing a bacteria culture, a thread or wire extending around said ampule and through said sterile closure in such manner that when said thread is pulled said ampule will contact the walls of said container and be broken whereby the contents of said ampule will be discharged into a substratum within said container, said ampule being composed of glass having such internal tensions that it will crush to dust when broken.

4. Container for the preservation of bacteria cultures, said container having a narrow mouth forming a shoulder and a sterile closure, an ampule within said container enclosing a bacteria culture, a thread or wire extending around said ampule .and through said sterile closure, the length of said ampule exceeding the internal width of the mouth of said container, the thread being of such length as to suspend the ampule in contact with the shoulders of said container so that when said thread is pulled said ampule will break against the shoulders of said container.

GUSTAV LARSEN.

REFERENCES CITED The following references are of record in the file of this patent:

UNITED STATES PATENTS Number Name Date 418,271 Buckmaster Dec. 31, 1889 1,332,985 Jarrett Mar. 9, 1920 1,991,938 Houghton Feb. 19, 1935 2,034,986 Mislowitzer Mar. 24, 1936 2,275,567 Smith Mar. 10, 1942 

1. A CONTAINER FOR THE PRESERVATION OF BACTERIA CULTURE COMPRISING A BOTTLE HAVING A NARROW NECK PORTION AND A STERILE CLOSURE, AN AMPULE WITHIN SAID BOTTLE ENCLOSING A BACTERIA CULTURE, SAID AMPULE HAVING AN EXTERNAL NARROW PASS IN THE MIDDLE THEREOF, A THREAD OR WIRE EXTENDING THROUGH SAID CLOSURE AND AROUND SAID NARROW PASS IN SUCH MANNER AS TO HOLD SAID AMPULE IN A TRANSVERSE POSITION NEAR THE NARROW NECK OF THE BOTTLE SO THAT WHEN SAID THREAD IS PULLED SAID AMPULE WILL BREAK AGAINST THE NECK OF SAID BOTTLE. 